Journal: Current biology : CB

Article Title: Evolutionary analysis of cellular reduction and anaerobicity in the hyper-prevalent gut microbe Blastocystis.

doi: 10.1016/j.cub.2023.05.025

Figure Lengend Snippet: Figure 3. Bioinformatic and molecular cell biological evidence for a minimal peroxisome in P. lacertae (A) Complement of Pex proteins in representative eukaryotes and selection of stramenopiles. Numbers in squares indicate counts of paralogous sequences. (B) Immunoblotting of anti-Pex19 (rabbit) and anti-Pex10 (rat) antibodies showing a strong band at 68 and 59 kDa, respectively (marked by asterisks).

Article Snippet: H. sapiens anti-Pex19 rabbit polyclonal antibody (ProteinTech Europe; 14713–1-AP).

Techniques: Selection, Western Blot

Journal: Experimental & Molecular Medicine

Article Title: Phosphodiesterase 4D contributes to angiotensin II-induced abdominal aortic aneurysm through smooth muscle cell apoptosis

doi: 10.1038/s12276-022-00815-y

Figure Lengend Snippet: a Immunofluorescence staining of PDE4D and α smooth muscle actin (α-SMA, smooth muscle cell marker) in human non-AAA sections and AAA tissues (6 μm). We obtained similar results from at least three different sets of human tissues in separate experiments. PDE4D: red, α-SMA: green. b Immunofluorescence staining of PDE4D and α-SMA in mouse control sections and AAA tissues (6 μm). We obtained similar results from at least three different sets of mouse samples in separate experiments. PDE4D: red, α-SMA: green. L: lumen. c RT-PCR analysis of Pde4d expression in rat aortic SMCs treated with Ang II (100 nM, 24 h). ** p < 0.0001, Welch’s t test, mean ± SEM, n = 3 separate experiments. d Representative immunoblot analysis of PDE4D protein expression in SMCs treated with Ang II (100 nM, 24 h). e Quantification of PDE4D protein expression by immunoblotting in ( d ) normalized to GAPDH protein (fold change versus control). ** p < 0.01, unpaired Student’s t test, mean ± SEM, n = 3 separate experiments.

Article Snippet: Immunoblotting of the housekeeping protein GAPDH (1:8000, Proteintech, US, Cat#: 60004-1-Ig) was performed to ensure equal protein loading.

Techniques: Immunofluorescence, Staining, Marker, Control, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot

Journal: Experimental & Molecular Medicine

Article Title: Phosphodiesterase 4D contributes to angiotensin II-induced abdominal aortic aneurysm through smooth muscle cell apoptosis

doi: 10.1038/s12276-022-00815-y

Figure Lengend Snippet: a Representative immunoblot analysis of caspase-3 and cleaved caspase-3 expression in the SMCs treated with PKI (PKA inhibitor, 10 μM, 60 min) and/or Ang II (100 nM, 24 h) and/or PDE4D siRNA (200 nM, 48 h) as indicated. b Quantification of cleaved caspase-3 protein expression by immunoblotting in ( a ) normalized to caspase-3 protein (fold change versus control). ** p < 0.01, *** p < 0.001, **** p < 0.0001, one-way ANOVA with Holm-Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. c Immunoblot analysis of caspase-3 and cleaved caspase-3 expression in the SMCs treated with ESI-09 (Epac inhibitor, 100 μM, 60 min) and/or Ang II (100 nM, 24 h) and/or PDE4D siRNA (200 nM, 48 h) as indicated. d Quantification of cleaved caspase-3 protein expression by immunoblotting in (c) normalized to caspase-3 protein (fold change versus control). **** p < 0.0001, ns: no significant difference, one-way ANOVA with Holm–Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. e Representative immunoblot analysis of phospho-Bad (pBad) and Bad expression in the SMCs treated with PKI (PKA inhibitor, 10 μM, 60 min) and/or Ang II (100 nM, 24 h) and/or PDE4D siRNA (200 nM, 48 h) as indicated. f Quantification of pBad expression by immunoblotting in ( e ) normalized to Bad protein (fold change versus control). ** p < 0.01, **** p < 0.0001, one-way ANOVA with Holm-Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. g Quantification of pBad expression by immunoblotting in ( e ) normalized to GAPDH protein (fold change versus control). ** p < 0.01, *** p < 0.001, one-way ANOVA with Holm–Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. h Quantification of Bad expression by immunoblotting in ( e ) normalized to GAPDH protein (fold change versus control). ** p < 0.01, *** p < 0.001, **** p < 0.0001, one-way ANOVA with Holm-Sidak’s post hoc test, mean ± SEM, n = 3 separate experiments. i PDE4D expression was significantly increased in Ang II-induced AAA, aggravating SMC apoptosis, which promoted pBad via the cAMP-PKA axis.

Article Snippet: Immunoblotting of the housekeeping protein GAPDH (1:8000, Proteintech, US, Cat#: 60004-1-Ig) was performed to ensure equal protein loading.

Techniques: Western Blot, Expressing, Control